Coding

Part:BBa_K1923011:Design

Designed by: Tianyang Mao   Group: iGEM16_Tsinghua   (2016-10-24)


Gal4BD-NLS-FLAG-EGFP-dCas9-NLS-Gal4AD encoding gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4673
    Illegal XhoI site found at 220
    Illegal XhoI site found at 567
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 139


Design Notes

This part is listed for the gold medal criterion (Improve the function OR characterization of a previously existing BioBrick Part or Device).


Source

This part was assembled from previous BioBricks (BBa_K1323002, BBa_K1493504 and BBa_K1470002) originating from distribution kits, and was integrated into a fusion protein.

References